Plasmid

Part:BBa_K4941036:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-pEYK1- Nluc-XPR2_terminator-ORI1001-Rep Origin (pMB1)-AmpR-Leu2 marker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4468
    Illegal XhoI site found at 4497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 5354
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 3883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3062
    Illegal BsaI site found at 4639
    Illegal BsaI.rc site found at 325
    Illegal BsaI.rc site found at 4554
    Illegal BsaI.rc site found at 4837
    Illegal SapI.rc site found at 4085


Design Notes

none


Source

yarrowia lipolytica and Lampyridae

References